International Journal of
MediPharm Research

In analytical chemistry, a calibration curve is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. Chemical methods are developed to fit particular business, regulatory and research purposes. The objective of this research paper is to set out principles which should be followed when documenting methods of chemical testing. The calibration curve of Amoxicillin trihydrate was plotted by using different solvents like distilled water, methanol, ethanol and dilute sodium hydroxide. Coefficient correlation (R2) of amoxicillin was found to be 0.999 in ethanol and less than 0.999 in methanol, distilled water and dil. sodium hydroxide.

Key words:Calibration curve, Amoxicillin trihydrate, Coefficient correlation.