International Journal of
MediPharm Research

ISSN 2395 - 423X

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Kajiram Adhikari
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Published and released Journal issue of 2021

Published and released Journal issue of 2021


Abstract

Photoproduction of Hydrogen by Anoxygenic Phototrophic Consortium Isolated from Pharmaceutical Industrial Effluents

Swetha Garimella, Vasavi Dathar, Vasantha Mittapelli, Ramchander Merugu

ABSTRACT

Hydrogen can be used as a clean fuel in future. Photoproduction of hydrogen by anoxygenic photosynthetic bacteria is efficient and ecofriendly. A study of hydrogen production (ml/ 10 ml vessel) was carried out using anoxygenic phototrophic consortium isolated from pharmaceutical industrial effluents with different carbon and nitrogen sources and various growth factors. The carbon source mannitol could induce a maximum of 2.5± 0.2 ml hydrogen followed by 2.0 ± 0.2 ml in benzoate, 2.0 ± 0.3 ml in acetate and 1.5 ± 0.3 in cellobiose containing medium. Ammonium chloride as nitrogen source could induced maximum of 3.0 ± 0.2 ml hydrogen and alanine induced lowest amount of hydrogen (I.0 ± 0.4 ml)by the photosynthetic consortium. Other nitrogen sources thiourea induced 2.5 ± 0.1 ml, valine 2.5 ± 0.2 ml and sodium nitrate 1.5 ± 0.2 ml of hydrogen. Of the growth factors tested, cyanocobalamine could induce highest amount of hydrogen (3.5 ± 0.1 ml) and niacin induced lowest amount (2.5 ± 0.4 ml) of hydrogen production. The growth factor riboflavin induced 3.0 ± 0.2 ml of hydrogen. Addition of growth factors showed an increase in hydrogen production by the phototrophic consortium. Highest production was seen in MAC medium at 2000lux at 1.5% concentration. Key words: Hydrogen production, PNSB, carbon, nitrogen.

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