International Journal of
MediPharm Research

The traditional Indian medicine such as Ayurveda, Siddha and Unani, describes usage of various herbs, fats, oils and minerals forinfected wound healing and management.The fruit of Phyllanthus emblicaLinn., also known as Amla is one of the most important components in the formulation of various Indiantraditional medicines such as Ayurveda, Unani and Siddha. The fruits of amla are useful in the treatment of various ailments such as diuretic, laxative, liver tonic, refrigerant, stomachic, restorative, anti-pyretic, hair tonic and ulcer preventive. The main bioactive constituent of amla is Ascorbic acid (Vitamin C) that is a co-factor in post translation modification of Collagen biosynthesis and poly phenols. Because of a rich in ascorbic acid in amla, we are motivated to evaluate the dermal and epidermal repair of infected wound in Wister albino rat and find the histological investigation of wound healing patterns. We found that In vivo studies and histological analysis of granulated tissue confirm almost >95% regeneration of dermis and epidermis in 16 days in treated groups of Wister Albino rats than that of open wound groups. The data on biochemical analysis of granulated tissue reveal that the newly synthesized components of extracellular matrix in the granulation tissue are more in treated group than open wound group. Moreover, the high amount of type 1 Collagen is synthesized in the granulation tissue of treated group and then better cross-linking of collagen in the granulation tissue was evaluated. Better maturation and cross-linking of Type 1 Collagen was observed in treated rats. Moreover, thealdehydes content of Type 1 Collagen in treated groups is also enhanced and confirmed that the better cross-linking of Type 1 collagen in the treated group. The preliminary investigation confirms that the methanol extract of amla has better wound healing properties in infected environment and useful medicine for wound management. Keywords: Methanol Extract of Amla, In vivo studies, Anti-microbial potential, Wound Pathogens and Type 1 Collagen synthesis and Aldehyde content and cross linking of Type 1 Collagen.