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Some of the pervoskite materials are exhibits perfect antibacterial effect, so they are appliedin surgical tool. Due to their non-toxic properties these perovskite coating materials may serve internal

 and external applications of human bodies. In this concern, here we report CaFexMn1-xO3-δ and BaFexMn1-xO3-δ (x = 0, 0.5 and 1) films on amorphous glass substrate synthesized by spin coating technique. The structural and morphological behaviour are identified by XRD and AFM analysis respectively. The crystallinity appeared only after annealing the films at 450°C. There is peak shifting takes place due to the addition of Fe3+ in Mn3+ ions. The existence of oxygen vacancy in complex elements is mainly due to the partial replacement of different elements on B-site, which strongly affects the oxygen vacancy. AFM analysis is taken under tapping mode, shows large numbers of spherical particles with the average particle size of 34 nm for BaFe0.5Mn0.5O3-δ. The antibacterial activity of CaFe0.5Mn0.5O3-δ and BaFe0.5Mn0.5O3-δ films are tested against two gram positive (Staphylococcus Aureus and Klebsiella Pneumoniae) and two gram negative (Pseudomonas and Escherichia Coli) bacteria. In which, CaFe0.5Mn0.5O3-δ is active against Pseudomonas and BaFe0.5Mn0.5O3-δfilm is active against Staphylococcus Aureus bacteria and their zone of inhibition is 23 mm and 18 mm respectively.