Research Article
Open Access
Evaluation for Total Phenolic, Total Flavonoid and Antioxidant activity of leaves and roots of Pyrus pashia
Preeti Rawat1*, Neetishwar Saroj2, Pinki Rawat3, Piyush Kumar4, Tryambak D Singh5, Mahesh Pal
1Phytochemistry Division, CSIR- National Botanical Research Institute, Lucknow, India.
2DJ College of Pharmacy, Modinagar, Gaziabad, India
3Dept. of Pharmaceutical Sciences and Technology, Birla institute of Technology, Ranchi,
Jharkhand, India
4Dept. of Chemistry, Indian Institute of Technology, Kanpur, India
5Dept. of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University,
Varanasi, India
Preeti Rawat et al. Evaluation for Total Phenolic, Total Flavonoid and Antioxidant activity of leaves and roots of Pyrus pashia. Int.J. MediPharm Res.2018,4(1),pp 48-51.
Abstract
The antioxidant activity and total phenolic content of methanolic extracts of leaves and fruits of Pyrus pashia were evaluated by using a model system containing DPPH free radical and Folin-Ciocalteu method. The total phenolic content of the extracts was determined spectrophotometrically according to the Folin-Ciocalteu procedure and found to be 325±10 mg/g and 98±5 mg/g in leaves and fruits respectively on fresh weight basis.The total flavonoid content of extracts of leaves and fruits were determined by Aluminium chloride colorimetric assay and ranged from 150±20 mg/g to 10.30±10 mg/g respectively.
Keywords
Pyrus pashia, Antioxidant, Phenolic, Flavonoid, DPPH
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