Research Article
Open Access
A Stability Indicating Validated Method for the Quantitation of hydrochlorothiazide by Using Diffuse Reflectance Infrared Fourier Transform Spectroscopy in bulk and tablet dosage form
S. J. Wadher1*, T. M. Kalyankar1, M. P. Puranik2 and Swami Jayshri1
1School of Pharmacy, Swami Ramanand Teerth Marathwada University, Vishnupuri,
Nanded-431606, Maharashtra, India
2Institute of Pharmaceutical Education and Research, Wardha, India
S. J. Wadher et al. A Stability Indicating Validated Method for the Quantitation of hydrochlorothiazide by Using Diffuse Reflectance Infrared Fourier Transform Spectroscopy in bulk and tablet dosage form. Int.J. MediPharm Res.2016,2(1),pp 32-41.
Abstract
A quantitative method using diffuse reflectance infrared fourier transform spectroscopy (DRIFTS) was developed and validated for the estimation of hydrochlorothiazide (HCTZ) in its tablet dosage form. The solid-state samples were, prepared by dilution in dry potassium bromide and were analyzed by FTIR spectrophotometer with DRIFT sampling technique. A linear relationship for the sulphone peak area centered on 1151 cm−1 was observed in the range of 1-6 % w/w with good correlation coefficient of 0.999. The percent recovery of hydrochlorothiazide in marketed tablet dosage form was in the range of 100 ± 0.3071. The present reported method is precise, reproducible, and eco-friendly. Diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) may have a potential as an alternative method for qualitative and quantitative analysis of hydrochlorothiazide in bulk drugs and tablet dosage forms.
Keywords
Hydrochlorothiazide, FTIR, Validation, Tablet, Infrared spectroscopy, Stability indicating method.
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