Antibacterial activity of Cardiospermum halicacabum was examined against clinical human bacterial pathogens. The butanol and methanol extract of C. halicacabum was endowed with plenty of phytochemical compounds. The susceptibility of Micrococcus luteus (33mm) was found pronounced followed by Proteus vulgaris as well as Staphylococcus aureus (32mm) against stem of acetone extract of C. halicacabum. The HPLC peaks at 2.267, 2.780 and 2.11 also describe the presence of active compounds, which are played key role in antimicrobial activity